pcr blunt ii topo invitrogen

pCR4-TOPO. There is a problem with the plasmid I received. Does Addgene accept orders by fax, phone or email? be delivered, each vial is about 5g. By continuing to use this site, you agree to the use of cookies. Today, Invitrogen TOPO PCR cloning kits are still among the fastest, simplest, and most flexible cloning solutions available. We appreciate your feedback. PDF pCR -Blunt II- TOPO - Thermo Fisher Scientific Zero Blunt TOPO PCR Cloning Kit, with pCR-Blunt II-TOPO Vector, One The maps, notes, and annotations in the zip file on this page are copyrighted material. Sign Up. Speed is less than 5000g. Electrophoresis, Western Blotting and ELISA, Chromatography and Mass Spectrometry Reagents, Laboratory Syringe Needles and Accessories, Lab Coats, Aprons, and Other Safety Apparel, Sharps Disposal Containers and Accessories, Classroom Laboratory Supplies and Consumables, Applied Biosystems TaqMan Assay and Arrays Search Tool, Applied Biosystems TaqMan Custom Assay Design Tools, Applied Biosystems Custom qPCR Primers and TaqMan Probes Tool, Chemical Storage and Management Resource Center, EcoRI sites flanking the PCR product insertion site for easy excision of inserts, Kanamycin and Zeocin resistance genes for your choice of selection in, SP6 promoter/primer site for in vitro RNA transcription and sequencing, M13 forward and reverse primer sites for sequencing or PCR screening, Fast and easy: go from PCR to clones in just three steps and in as little as 5 minutes hands-on time, Efficient: obtain up to 95% clones with correct insert, Proven: reliable performance for over a decade with over 4,000 citations, Simple: no ligase, post-PCR procedures, or PCR primers containing specific sequences are required, Vector: pCRBlunt II-TOPO vector: contains the, Cloning method: TOPO cloning: Topoisomerase Ibased, 5-minute ligation of blunt-ended PCR products amplified with proofreading thermostable polymerases, Competent cells: Various options: choose from kits with either general, high-efficiency, bacteriophage T1-resistant, fast-growing competent cells (available separately) or use your own, Antibiotic resistance: Ampicillin (AmpR), Kanamycin (KanR). What is virus associated DNA, and why do I have to order it. I have used 1 ul of 10 ng/ul insert and 1 ul of TOPO vector. The Zero Blunt TOPO PCR Cloning Kit for Subcloning, without competent cells allows the flexibility of using your own strain for increased flexibility so competent cells and S.O.C. What strain of bacteria does my stab contain? Consortium Plasmids|More Plasmid Sets No matches HomePlasmidsI.M.A.G.E. Each Zero Blunt TOPO for subcloning Kit contains linearized and topoisomerase I-activated pCR Blunt II-TOPOVector, Salt Solution, dNTPs, Control Template and Primers, M13 Forward and Reverse Primers, One Shot Chemically Competent or ElectrocompE. coli, S.O.C. The pCRBlunt II-TOPO vector used in this kit comes with lethal ccdB gene for efficient selection of blunt-ended PCR recombinants products generated by thermostable proofreading polymerases. Plasmid Lox-Stop-Lox TOPO from Dr. Tyler Jacks's lab contains the insert loxP-STOP-loxP and is published in Genes Dev. pCR-Blunt II-TOPO Benchling pCR-Blunt II-TOPO (linearized) Sequence and Map - SnapGene The - pCR-Blunt vector contains the lethal E. coli ccdB gene fused to the C-terminus of LacZ (Bernard et al., 1994).Ligation of a blunt PCR fragment disrupts expression Bioz Stars score: 86/100, based on 1 PubMed citations. Contact Addgene. In addition, a Zero Blunt TOPO Cloning Kit is available combined with a Invitrogen PureLink Quick Plasmid Miniprep Kit (50 preps) for fast plasmid purification of your TOPO-cloned inserts for downstream analysis. pCRBlunt II-TOPO vector: designed for proofreading polymerasesThe pCRBlunt II-TOPO vector is designed to clone blunt-ended PCR products generated by thermostable proofreading polymerases such as Platinum Pfx DNA Polymerase. Addgene - Analyze Sequence Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. Kit includes linearized and topoisomerase I-activated pCR-Blunt II-TOPO Vector for 5-minute benchtop ligations without ligase. Thermo Fisher Scientific, Zero Blunt TOPO PCR Cloning Kit provides a highly efficient, 5-minute, one-step cloning strategy ("TOPO Cloning") for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector. Invitrogen Zero Blunt TOPO technology provides a highly efficient, 5-minute, one-step cloning strategy for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector. Receive the latest news, hot plasmids, discounts and more. The standard in cloning When it comes to cloning, TOPO cloning technology has been a reliable partner for thousands of scientists for over ten years. & Engineering, Model What is an MTA/Who is authorized to sign? Genome Analyze Sequence: pCR blunt II TOPO. If you run into any problems registering, depositing, or ordering please contact us at [emailprotected] No ligase, post-PCR procedures, or PCR primers containing specific sequences are required. Pcr Blunt Ii Topo Backbone Vector | Thermo Fisher | Bioz Please sign in to view account pricing and product availability. 1333 Xinlong Road, Minhang District, Shanghai, China, Zip-code: 201101, Copyright 2014 NovoPro Bioscience Inc. All rights reserved. pCR-Blunt II-TOPO 3519 bp 500 1000 1500 2000 2500 3000 3500 BsaXI, AseI, +25 +4 KasI SphI NcoI, +3 +9 +1 +3 +5 +1 AlwNI BssSI, +1 DrdI, AflIII, This is a free resource for the scientific community that is compiled by Addgene. Systems, Research Sequence Author: Thermo Fisher (Invitrogen) Open in SnapGene. Medium are not provided. Two vials of lyophilized plasmid will However, we are committed to improving your shopping experience. Welcome to Vector Database! The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. Eco R I sites flanking the PCR product insertion site for easy excision of inserts. All contact information provided shall also be maintained in accordance with our The pCR Blunt II-TOPO vector is designed to clone blunt-ended PCR products generated by thermostable proofreading polymerases such as Platinum SuperFi DNA Polymerase. Includes the Zero Blunt TOPO vector containing the ccdB gene for positive selection, only permitting growth of plasmid vectors with recombinants. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as www.snapgene.com/resources. 2001 Dec 15. I have even tried to increase the insert (2:1 . PDF TOPO PCR cloning solutions - Thermo Fisher Scientific 2. Your feedback has been submitted. pCR-Blunt II-TOPO (linearized) Linearized vector for TOPO cloning of blunt PCR products. Systems, Research 4. This website uses cookies to ensure you get the best experience. 11. Learn about the latest plasmid technologies and research tools. Not for use in diagnostic procedures. pCR-Blunt II-TOPO vector map and sequence - novoprolabs.com Have questions about your order, deposit, or a plasmid? pCR-Blunt II-TOPO Sequence and Map - SnapGene Zero Blunt TOPO PCR Cloning Kit, without competent cells Copyright 2006-2023 Thermo Fisher Scientific Inc. All rights reserved, Zero Blunt TOPO PCR Cloning Kit, with pCR-Blunt II-TOPO Vector, One Shot TOP10 Chemically Competent, Spectroscopy, Elemental and Isotope Analysis. Not for use in diagnostic procedures. Each kit has the Zero Blunt TOPO vector (see figure) containing the, Blunt DNA Cloning Vectors, TOPO Cloning Vectors, pCR, Copyright 2006-2023 Thermo Fisher Scientific Inc. All rights reserved, Zero Blunt TOPO PCR Cloning Kit, without competent cells, Spectroscopy, Elemental and Isotope Analysis. Consortium PlasmidspCR-Blunt II-TOPO The pCRBlunt II-TOPO vector contains: The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. Please use the form below to provide feedback related to the content on this product. Learn more, Please note: Your browser does not fully support some of the features used on Addgene's website. The pCRBlunt II-TOPO vector contains: EcoRI sites flanking the PCR product insertion site for easy excision of inserts PDF TOPO TA Cloning - Molbio Simple. Press Release Team Advisors Partners Contact Bioz Stars Bioz vStars 86 Buy from Supplier Structured Review Thermo Fisher pcr blunt ii topo backbone vector Pcr Blunt Ii Topo Backbone Vector, supplied by Thermo Fisher, used in various techniques. no.K2800-02) is shipped with an additional box containing reagents for plasmid purification (Box 3). & Engineering, Model Citing NovoPro. The Zero Blunt PCR Cloning Kit offers an easy method for high-efficiency (>80%) cloning of blunt-end PCR products amplified with proof-reading, thermostable DNA polymerases. Molecular Genetics TOPO Vector Cloning Vector Human Molecular Genetics Most recent answer Magnus Wohlfahrt Rasmussen University of Copenhagen 11th Apr, 2015 That's true, you can make a regular. Privacy Policy. What is an MTA/Who is authorized to sign? Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Room 229, Building 26, No. Have questions about your order, deposit, or a plasmid? Les kits cellulaires comptents contiennent des cellules d, Copyright 2006-2023 Thermo Fisher Scientific Inc. All rights reserved, Colonnes et cartouches de chromatographie, Consommables en plastique et fournitures de laboratoire, Afficher toutes les catgories de produits, Spectroscopie, analyse lmentaire et isotopique, Voir toutes les applications et techniques, Services aux organisations de dveloppement et de fabrication sous contrat (CDMO) et pour les essais cliniques, Consultez toutes les rubriques d'aide et d'assistance. Please sign in to view account pricing and product availability.
What Is Aspen Bedding Made Of, An Article In A Company Newsletter, What Age Group Uses Tiktok The Most, Mongodb Database Size In Gb, Graphic Pickguard Strat, Articles P